BeNa Culture Collection
Culture medium | LB + 50mcg/ml ampicillin: yeast extract 5.0g, peptone 10.0g,NaCl 10.0g, distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. After sterilization, ampicillin was added when the culture medium was cooled to 40-50 ℃ with a concentration of 50mcg/ml. |
Subculture procedure | null |
Growth conditions | LB + Ampicillin, 37 ℃ (Cloned Strain DH5α,5741bp) |
Storage conditions | 2-8 ℃ |
Safety level | 0 |
application | Scientific research |
Sharing mode | Public welfare sharing |