Mycobacterium colombiense DNA lab quality control|BNCC372397 |BNCC

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Mycobacterium colombiense DNA lab quality control

Culture medium	Ammonium sulfate: 0.5, potassium dihydrogen phosphate: 1.5, disodium hydrogen phosphate: 1.5, sodium citrate: 0.4, magnesium sulfate: 0.025, calcium chloride: 0.5mg, zinc sulfate: 0.001, copper sulfate: 0.001, sodium L-glutamate: 0.5, iron ammonium citrate: 0.04, pyridoxine hydrochloride: 0.001, biotin: 0.5mg, malachite green: 0.25mg, agar: 15.0,PH:6.4-6.8(25 ℃)
Subculture procedure	Remove from the refrigerator, melt completely, vortex and mix well, ready to use. Note: The quality control is inactivated particles, which need to be used after nucleic acid extraction. The extraction or purification reagents should be prepared by themselves, and repeated freezing and thawing should be avoided before extraction. The minimum sampling volume is 200 μl.
Growth conditions	37 ℃;3-7 days; aerobic;
Storage conditions	-80 ℃
Safety level	2
Separation substrate	Human Blood, AIDS Patients
application	The quality control is developed by Henan engineering center of industrial microbial strain, which is the inactivated virus suspension of Mycobacterium Columbia, and can be used for method establishment, working standard assignment, method confirmation and evaluation, product quality control, etc. by digital PCR value.
Sharing mode	Public welfare sharing