Lactobacillus gasseri DNA standard|BNCC368139 |BNCC

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Lactobacillus gasseri DNA standard

Culture medium	Peptone: 10.0, beef extract powder: 8.0, yeast powder: 4.0, glucose: 20.0, magnesium sulfate: 0.2, sodium acetate: 5.0, diammonium citrate: 2.0, dipotassium hydrogen phosphate: 2.0, manganese sulfate: 0.04, tween 80:1.0, agar: 14.0,pH:5.7±0.2(25 ℃)
Subculture procedure	When using this standard, move it to room temperature to be completely thawed, shake evenly, and use it after instantaneous centrifugation. Try to avoid repeated freezing and thawing.
Growth conditions	37 ℃;24-48h;5% CO2
Storage conditions	-80 ℃
Safety level	1
Separation substrate	human intestine
application	This Lactobacillus DNA standard can be used for method establishment, working standard assignment, method confirmation and evaluation, product quality control, etc. by fluorescence quantitative pcr combined with digital PCR.
Sharing mode	Public welfare sharing