Mycobacterium scrofulaceum DNA standard|BNCC364899 |BNCC

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Mycobacterium scrofulaceum DNA standard

Culture medium	Ammonium sulfate: 0.5, potassium dihydrogen phosphate: 1.5, disodium hydrogen phosphate: 1.5, sodium citrate: 0.4, magnesium sulfate: 0.025, calcium chloride: 0.5mg, zinc sulfate: 0.001, copper sulfate: 0.001, sodium L-glutamate: 0.5, iron ammonium citrate: 0.04, pyridoxine hydrochloride: 0.001, biotin: 0.5mg, malachite green: 0.25mg, agar: 15.0,PH:6.4-6.8(25 ℃)
Subculture procedure	① Prepare 2 pieces of the above plates; ② Open the surface of the ampoule tube in a safety cabinet after disinfection, burn the top with an alcohol lamp, quickly drop sterile water to break it, and then crush it with tweezers; ③ Absorb 0.5mL sterile water and freeze-dry it into the tube, fully dissolve the bacterial powder, then put it into 2 plates, 200 μL/piece, and coat it evenly; ④ Place the plate under the above culture conditions, and the bacteria grow out.
Growth conditions	30 ℃;3-5 days; aerobic
Storage conditions	2-8 ℃
Safety level	2
morphology	The colony is 0.5-1mm in diameter, round, with neat edges, opaque, gray-white front, convex in the middle, smooth surface, bright surface, moist texture, easy to pick up, G + (blue-purple), bacilli, purity: pure
application	The quality control is developed by Henan engineering center of industrial microbial strain, which is suitable for qualitative and quantitative research and analysis of Mycobacterium avium, verification and evaluation of measurement methods and laboratory quality control.
Sharing mode	Public welfare sharing