Lactobacillus casei DNA standard|BNCC364045 |BNCC

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Lactobacillus casei DNA standard

Culture medium	Peptone: 10.0, beef extract powder: 8.0, yeast powder: 4.0, glucose: 20.0, magnesium sulfate: 0.2, sodium acetate: 5.0, diammonium citrate: 2.0, dipotassium hydrogen phosphate: 2.0, manganese sulfate: 0.04, tween 80:1.0, agar: 14.0,pH:5.7±0.2(25 ℃)
Subculture procedure	When using this standard, move to room temperature to be completely dissolved, shake evenly, use after instantaneous centrifugation, try to avoid repeated freezing and thawing.
Growth conditions	37 ℃,24-48h, facultative anaerobic
Storage conditions	-80 ℃
Safety level	1
application	The lactobacillus casei DNA standard, by fluorescence quantitative pcr combined with digital PCR value, can be used for method establishment, working standard assignment, method confirmation and evaluation, product quality control, etc.
Sharing mode	Public welfare sharing