BeNa Culture Collection
Culture medium | Tryptone: 15.0, soy peptone: 5.0, sodium chloride: 5.0, sterile defibrated sheep blood: 5%, agar: 15.0,pH:7.3±0.2(25 ℃) |
Subculture procedure | ① Prepare 1-2 pieces of the above-mentioned plates (deoxygenate in anaerobic environment for 24 hours in advance);② Open the safety cabinet, burn the top with alcohol lamp, quickly drop sterile water to break it, and then crush it with tweezers; ③ Absorb 0.5mL sterile water into freeze-drying tube, fully dissolve the bacterial powder, and then put it into the plate at 200 μL/piece, and coat it evenly. ④ Place the plate under the above culture conditions, and the strain can be used. |
Growth conditions | 37 ℃;24-48h; anaerobic; |
Storage conditions | 2-8 ℃ |
Safety level | 2 |
morphology | The colony diameter is 2-4mm, round, irregular edges, opaque, white on the front, raised in the middle, smooth surface, bright surface, moist texture, easy to provoke, G-(red), bacilli, purity: pure |
Sharing mode | Public welfare sharing |