Mycobacterium shimoidei DNA lab quality control|BNCC368036 |BNCC

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Mycobacterium shimoidei DNA lab quality control

Culture medium	Ammonium sulfate: 0.5, potassium dihydrogen phosphate: 1.5, disodium hydrogen phosphate: 1.5, sodium citrate: 0.4, magnesium sulfate: 0.025, calcium chloride: 0.5mg, zinc sulfate: 0.001, copper sulfate: 0.001, sodium L-glutamate: 0.5, iron ammonium citrate: 0.04, pyridoxine hydrochloride: 0.001, biotin: 0.5mg, malachite green: 0.25mg, agar: 15.0,PH:6.4-6.8(25 ℃)
Subculture procedure	Remove from the refrigerator, melt completely, vortex and mix well, ready to use. Note: The quality control is inactivated particles, which need to be used after nucleic acid extraction. The extraction or purification reagents should be prepared by themselves, and repeated freezing and thawing should be avoided before extraction. Minimum sampling volume of 200 μl
Growth conditions	37 ℃;10-15 days; aerobic;
Storage conditions	-80 ℃
Safety level	2
Separation substrate	Sputum in patients with tuberculosis-like disease
application	The quality control is developed by Henan engineering center of industrial microbial strain, which is obtained by adding stabilizers and digital PCR fixed values, and can be used for method establishment, work standard assignment, method confirmation and evaluation, product quality control, etc.
Sharing mode	Public welfare sharing