Escherichia coli EHEC DNA lab quality control|BNCC366466 |BNCC

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Escherichia coli EHEC DNA lab quality control

Culture medium	Peptone: 10.0, beef powder: 3.0, sodium chloride: 5.0, agar: 15.0,pH: 7.3±0.1(25 ℃)
Subculture procedure	When using this standard, move to room temperature to be completely dissolved, shake evenly, use after instantaneous centrifugation, try to avoid repeated freezing and thawing. Use after extracting nucleic acid according to the instructions of nucleic acid extraction reagent. The nucleic acid extraction or purification reagent shall be prepared by itself. The minimum sampling amount is 200 μL.
Growth conditions	37 ℃;18-24h; aerobic;
Storage conditions	-80 ℃
Safety level	2
application	The quality control is developed by Henan engineering center of industrial microbial strain, which is the inactivated bacterial suspension of enterohemorrhagic Escherichia coli, and can be used for method establishment, working standard assignment, method confirmation and evaluation, product quality control, etc. by digital PCR value.
Sharing mode	Public welfare sharing