Clostridium sporogenes DNA lab quality control|BNCC365119 |BNCC

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Clostridium sporogenes DNA lab quality control

Culture medium	Peptone (trypsin hydrolysis):15.0, yeast extract powder: 5.0, glucose: 5.0, sodium thioglycolate: 0.5,L-cystine: 0.5, sodium chloride: 2.5, resazurin: 0.001, agar: 0.75,pH: 7.1±0.2(25 ℃)
Subculture procedure	Remove from the refrigerator, melt completely, vortex and mix well, ready to use. Note: The quality control is inactivated particles, which need to be used after nucleic acid extraction. The extraction or purification reagents should be prepared by themselves, and repeated freezing and thawing should be avoided before extraction. The minimum sampling volume is 200 μl.
Growth conditions	37 ℃,18-24h, anaerobic
Storage conditions	-80 ℃
Safety level	2
application	The quality control is developed by Henan engineering center of industrial microbial strain, and is obtained by adding stabilizers and digital PCR fixed values, which can be used for method establishment, working standard assignment, method confirmation and evaluation, product quality control, etc.
Sharing mode	Public welfare sharing