Mycobacterium chelonae DNA standard|BNCC363841 |BNCC

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Mycobacterium chelonae DNA standard

Culture medium	Nutritional gravy medium (NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (without liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid culture medium and 2 plates; (2) Open the safety cabinet, burn the top with an alcohol lamp, quickly drop sterile water to break it, and then crush it with tweezers; (3) Absorb 0.5mL of liquid culture medium into a freeze-drying tube, fully dissolve and return to the liquid test tube, and mix well; (4) Absorb 0.2mL of bacteria suspension into the plate and coat it evenly, repeat twice to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow out.
Growth conditions	37 ℃,24-48h, aerobic
Storage conditions	2-8 ℃
Safety level	2
morphology	The colony diameter is 1-2mm, round, with neat edges, opaque, light color, convex in the middle, smooth surface, bright surface, moist texture, easy to pick up, no pigment, G + (blue-purple), bacilli, purity: pure
Sharing mode	Public welfare sharing