Corynebacterium ulcerans nucleic acid reference (Heat inactivated) (Strongly positive)|BNCC361626 |BNCC

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Corynebacterium ulcerans nucleic acid reference (Heat inactivated) (Strongly positive)

Culture medium	Brain heart extract medium (English name: BHIA/BHIB): brain extract 5.0g, heart extract 12.5g, peptone 5.0g, tryptone 10.0g, sodium chloride 5.0g, glucose 2.0g, disodium hydrogen phosphate 2.5g, agar 20.0g (not included in liquid medium),pH 7.4±0.2. Sterilization at 121 ℃ for 15min.
Subculture procedure	Before use, the reference product is placed at room temperature for 30min, fully melted, shaken and mixed, used after nucleic acid extraction, nucleic acid extraction or purification reagents need to be provided by oneself.
Growth conditions	Culture temperature 37 ℃; Culture time 18~24 hours; Aerobic gas environment;
Storage conditions	-80 ℃
Safety level	1
application	Accurate and rapid identification of pathogenic bacteria can effectively improve the pertinence and effectiveness of clinical treatment. This product uses heat-inactivated strong positive reference materials for the evaluation of the nucleic acid detection reagent of Corynebacterium ulcerans for early diagnosis and quality control of infection.
Sharing mode	Public welfare sharing

Certificate of Corynebacterium ulcerans nucleic acid reference product

1. Product information

Sample name: Corynebacterium ulcerans nucleic acid reference material (heat inactivated) (strong positive)

Sample number: 361626

Sample batch: 220523

2. Product features

Description	Packaged in 1.5mL cryovials, containing 0.5mL nucleic acid reference, in liquid state
Preparation method	inoculate broth medium of brain-heart leachate, PBS suspension, 80 ℃ water bath inactivation for 30min
Concentration standard	≥10⁷CFU/mL	Reference value	3.5×10⁷CFU/mL
Authentication	colony morphology is consistent with corynebacterium ulcerans	results	match
*Note: If you have any questions about the reference material, please contact our center (BNCC) for help before use

3. Instructions

Before use, place the reference material at room temperature for 30 minutes, fully thaw, shake and mix well, and use it after extracting nucleic acid. Nucleic acid extraction or purification reagents should be prepared by yourself.

4. Notes

1. After receiving the goods, please be sure to keep them properly. If there is any damage, please contact the center within 24 hours after receipt;

2. This product uses the heat inactivation method, which is likely to destroy the protein antigen, and the test related to the protein antigen is not applicable;

3. It should be used as soon as possible after thawing to avoid repeated freezing and thawing more than 3 times;

4. In the process of use, it should be handled according to infectious substances. Please do not touch the samples in the bottle directly with your hands. After use, please sterilize the residual samples at high temperature and high pressure and discard them;

5. Preservation and transportation

Long-term storage:-80 ℃, valid for 2 years; Transportation conditions: dry ice transportation.

Henan Engineering Technology Research Center of Industrial Microbial Strain

website: www.bncc.org.cn tel: 400-6699-833

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