BeNa Culture Collection
Subculture procedure | This reference does not recommend repeated freezing and thawing. Before use, take it out of the refrigerator, balance to room temperature, mix well, and use it. |
Storage conditions | -80 ℃ |
Safety level | 1 |
application | Validation and evaluation of qualitative and quantitative measurement methods for novel coronavirus mutant genes and laboratory quality control. |
Sharing mode | Public welfare sharing |
New coronavirus B .1.1.7 mutant nucleic acid liquid quality control
The essential control is the nucleocapsid protein gene ribonucleic acid of the new coronavirus B .1.1.7 mutant, which is used for the verification and evaluation of qualitative and quantitative measurement methods of the N gene of the new coronavirus mutant and laboratory quality control.
1. product information
product name | new coronavirus B .1.1.7 mutant nucleic acid liquid quality control | ||
description | there are 20 pieces of this product, each containing 0.5mL, frozen | ||
number | BNCC 361558 | batch | 220516 |
store | -80℃ | validity period | 2 years |
2. product features
historical source | Henan industrial microbial strain engineering technology research center & larr; | |
preparation method |
RNA transcripts of nucleocapsid protein N gene (full length), an important characteristic gene of the novel coronavirus mutant, were obtained through in vitro transcription (including the two mutation sites of D3L and S235F of the N gene), diluted to a certain concentration. |
|
fixed value method | The absolute quantitative method-digital PCR method was used to determine the copy number concentration of the mutant N gene to obtain the standard value of the standard substance. | |
attribute value | specifications (copies/mL) | reference value (copies/mL) |
103~104 | 2.3×103 |
3. instructions
This reference product is not recommended for repeated freezing and thawing. Before use, take it out of the refrigerator, equilibrate to room temperature, mix well, and it is ready to use.
4. notes
1. After receiving the goods, if any damage is found, please contact the center in time within 24 hours, and reissue it free of charge after confirmation;
2. When using this standard material for measurement, please carefully verify the gene sequence, especially when measuring genes containing mutation sites, the primers and probe sequences used should match. Gene sequences can be provided free of charge according to user needs.
Henan Engineering Research Center of Industrial Microbiology
Website: www.bncc .org .cn Tel: 400-6699-833