Hemorrhagic Escherichia coli O157:H7 nucleic acid reference material (Heat inactivated)(Strongly positive)|BNCC360677 |BNCC

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Hemorrhagic Escherichia coli O157:H7 nucleic acid reference material (Heat inactivated)(Strongly positive)

Culture medium	Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculture procedure	Before use, balance the reference at room temperature for 30min and thaw. Equilibrium to room temperature, vortex oscillation and mixing, according to the nucleic acid extraction reagent instructions to extract nucleic acid after use, nucleic acid extraction or purification reagents need to be self-provided.
Storage conditions	-80 ℃, kept away from light
Safety level	2
application	Accurate and rapid identification of pathogenic bacteria can effectively improve the pertinence and effectiveness of clinical treatment. This product uses heat inactivated strong positive reference for the evaluation of hemorrhagic Escherichia coli O157:H7 nucleic acid detection reagent for early diagnosis and quality control of infection.
Sharing mode	Public welfare sharing

Hemorrhagic Escherichia coli O157:H7 Nucleic Acid Reference Certificate

1. Product information

Sample name: Hemorrhagic Escherichia coli O157:H7 Nucleic Acid Reference (Heat Inactivated)

Sample number: 360677

sample batch: 220208

2. Product features

Description	1.5mL frozen storage tube package, containing 0.5mL nucleic acid reference, is transparent liquid
Traceability	CICC 21530 hemorrhagic Escherichia coli O157:H7
Concentration value	≥10⁶CFU/mL
Applicable method	Related research on nucleic acid detection reagents for pathogens
*Note: If you have any questions about the reference material, please contact our center (BNCC) for help before use

3.Instructions

Before use, place the reference substance at room temperature for 30 minutes, fully thaw, shake and mix well, and use it after extracting nucleic acid. The nucleic acid extraction or purification reagents should be prepared by yourself.

4. Notes

1. After receiving the goods, please be sure to keep them properly. If there is any damage, please contact the center within 24 hours after receipt;

2. This product uses the heat inactivation method, which is likely to destroy the protein antigen, and the test related to the protein antigen is not applicable;

3. It should be used as soon as possible after thawing to avoid repeated freezing and thawing more than 3 times;

4. In the process of use, it should be treated according to infectious substances. Please do not directly touch the samples in the bottle with your hands. After use, please sterilize the residual samples at high temperature and high pressure and discard them;

5. Preservation and transportation

Long-term storage:-20 ℃, valid for 2 years; Transportation conditions: dry ice transportation.

Henan Engineering Technology Research Center of Industrial Microbial Strain

website: www.bncc.org.cn tel: 400-6699-833