Streptococcus salivarius nucleic acid reference (Heat inactivated)(Strongly positive)|BNCC360611 |BNCC

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Streptococcus salivarius nucleic acid reference (Heat inactivated)(Strongly positive)

Culture medium	Brain heart extract medium (English name: BHIA/BHIB): brain extract 5.0g, heart extract 12.5g, peptone 5.0g, tryptone 10.0g, sodium chloride 5.0g, glucose 2.0g, disodium hydrogen phosphate 2.5g, agar 20.0g (not included in liquid medium),pH 7.4±0.2. Sterilization at 121 ℃ for 15min.
Subculture procedure	Before use, balance the reference at room temperature for 30min and thaw. Equilibrium to room temperature, vortex oscillation and mixing, according to the nucleic acid extraction reagent instructions to extract nucleic acid after use, nucleic acid extraction or purification reagents need to be self-provided.
Growth conditions	37 ℃;18-24h; Aerobic
Storage conditions	-80 ℃, kept away from light
Safety level	1
application	Accurate and rapid identification of pathogenic bacteria can effectively improve the pertinence and effectiveness of clinical treatment. This product uses heat inactivated strong positive reference for the evaluation of Streptococcus salivarius nucleic acid detection reagent, in order to be used for early diagnosis and quality control of infection.
Sharing mode	Public welfare sharing

Streptococcus salivarius nucleic acid reference certificate

1. Product information

Sample name: Streptococcus salivarius nucleic acid reference (heat inactivated)

Sample number: 360611

sample batch: 220107

2. Product features

Description	Appearance is transparent liquid, packaged in 1.5mL cryovial
Traceability	GIM1.949 Streptococcus salivarius
Concentration value	≥10^6CFU/mL
Uniformity and stability	Random sampling, the uniformity of the results is good; long-term stability test, the stability is qualified
Applicable method	Related research for pathogen nucleic acid detection reagents
*Note: If you have any questions about the reference material, please contact our center (BNCC) for help before use

3. Instructions

Before use, the reference material was equilibrated at room temperature for 30 minutes and thawed. Equilibrate to room temperature. After vortexing and mixing, extract the nucleic acid according to the instructions of the nucleic acid extraction reagent before use. The nucleic acid extraction or purification reagent should be prepared by yourself.

4. Notes

1. After receiving the goods, please keep them properly. If there is any damage, please contact the center within 24 hours after receipt;

2. This material is inactivated by heat inactivation, which is likely to cause damage to protein antigens, and is not suitable for experiments related to protein antigens;

3. The research materials should be used as soon as possible after freezing and thawing, and can be stored at -80°C and below, but avoid repeated freezing and thawing more than 3 times;

4. During the use of this research material, it should be treated as infectious substances. Please do not touch the samples in the bottle directly with your hands. After use, please sterilize the residual samples at high temperature and high pressure and discard them;

5. Preservation and transportation

long-term storage:-80 ℃, valid for 2 years; Transportation conditions: dry ice transportation, the time should not exceed 7 days.

Henan Engineering Technology Research Center of Industrial Microbial Strain

website: www.bncc.org.cn tel: 400-6699-833