BeNa Culture Collection
Culture medium | Nutritional gravy medium (NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (without liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mg MnSO4 · H2O to culture Bacillus is beneficial to produce spores. |
Subculture procedure | (1) According to the culture conditions, the bacteria are first cultured into a bacterial suspension; ② Measure the absorbance of the bacterial suspension and calculate the initial concentration of the bacterial suspension according to the standard formula; (3) The initial concentration multiplied by the survival rate is the actual concentration after freeze-drying; (4) Dilute the bacterial suspension according to the actual concentration after freeze-drying to obtain the target concentration, and then subpack and freeze-dry; |
Growth conditions | 30 ℃, aerobic |
Storage conditions | -80 ℃ |
morphology | Purity: pure |
application | Kauffmann serotype 23ac 12a |
Sharing mode | Public welfare sharing |
Proteus sp.
Strain number: BNCC 356096 / CMCCB49027
Name: proteus Proteus sp.
Product format: quantitative bacteria tablets Number of viable bacteria: 5.0~6.5×108CFU/bottle
Product batch: 201204 Valid until: 2021.12.03
Date of inspection: 2020.12.05 Sampling ratio: ≥2%
Report number: RS20356096-1 Biosafety level: level 2, handle in safety cabinet
project | description | results |
stain microscopy | gram negative and positive, cell morphology observed under microscope | G-bacilli, in a single rod shape |
colony morphology | observation of single colony morphology | milky white, raised, bright, or spread |
Purity | non-selective medium culture, colony observation and microscopic examination | No contaminated bacterial |
Reference value of live bacteria | Non-selective medium plate, viable count | 5.7×108CFU/bottle |
Phenotypic and Genotypic Analysis | phenotypic testing, gene sequencing | qualified |
test conclusion | qualified |
Product list: 5.0~6.5×108CFU quantitative strain 1 bottle, 1mL dissolved solution 1 bottle, strain certificate 1 copy;
Shelf life: unopened, below -20 ℃, 12 months;
Usage:
1. Open the strain and dissolved solution according to the arrow of Xilin bottle cap;
2. Pour the bacteria tablets into the dissolved liquid, cover the rubber stopper, shake and dissolve;
3. after full dissolution, it is 5.0~6.5×108CFU/mL, which can be sucked in proportion according to the test requirements;
4. Please use up immediately after opening, and do not store it;
Notes:
① This product is a quantitative strain. improper storage conditions and usage methods will affect the accuracy of the number of strains, so please strictly follow the above storage conditions and methods.
② Used bacteria-carrying consumables, please autoclave and discard them.