BeNa Culture Collection
Culture medium | Brain heart extract medium (BHIA/BHIB): brain extract 5.0g, heart extract 12.5g, peptone 5.0g, tryptone 10.0g, sodium chloride 5.0g, glucose 2.0g, disodium hydrogen phosphate 2.5g, agar 20.0g (without liquid medium),pH 7.4±0.2. Sterilization at 121 ℃ for 15min. |
Growth conditions | Temperature: 30°C Atmosphere: Aerobic |
Storage conditions | -20 ℃ |
Sharing mode | Public welfare sharing |
Bacillus subtilis
No.:353859 / ATCC 6633
Product format: quantitative bacteria tablets
Number of viable bacteria : 0.5~1.0×10 4CFU/bottle
Product batch: 191227
Valid until : 2020.12.26
Date of inspection: 2019.12.29
Sampling ratio : ≥2%
Report number : RS19353859-1
Biosafety level : 1 , handle in ultra-clean table or safety cabinet
item | description | test result |
stain microscopy | Gram-positive, observe cell morphology under microscope | G + cocci |
colony morphology | observation of single colony morphology | Gray, dry, flat, irregular edges |
purity | non-selective medium culture, colony observation and microscopic examination | No bacteria |
Reference value of live bacteria | non-selective medium plate, live bacteria count | 9040CFU/bottle |
phenotype and genotype analysis | phenotypic testing, gene sequencing | qualified |
test conclusion | after quality inspection by BNCC quality control testing system, the product is qualified |
Product list :One bottle of 0.5~1.0×10 4CFU quantitative strain, one bottle of 1mL dissolved solution and one copy of strain certificate;
Validity period: unopened, below -20 ℃, 12 months;
Handling procedure:
1 Open the penicillin-bottle according to the arrow indication on the vial cap;
2 Transfer the strain tablets into the vial of solution, capped with rubber plug, and shake to dissolve
3 It will be 0.5~1.0x10 4 cfu/ml after full dissolved, and use appropriate amount according to the operation demand
4 The bacterial tablets shall be used up once and shall not be retained once the penicillin bottled is opened.
Notes:
1 This product is a quantitative strain. Improper storage conditions and handling procedure will affect the accuracy of the strain quantities. Therefore, please operate in strict accordance with the above mentioned instructions.
2 Waste generated from the handling process should be discarded after high-pressure sterilization