Quantitative strain of Bacillus subtilis|BNCC353859 |BNCC

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Bacillus subtilis subsp. spizizenii Nakamura et al.

Culture medium	Brain heart extract medium (BHIA/BHIB): brain extract 5.0g, heart extract 12.5g, peptone 5.0g, tryptone 10.0g, sodium chloride 5.0g, glucose 2.0g, disodium hydrogen phosphate 2.5g, agar 20.0g (without liquid medium),pH 7.4±0.2. Sterilization at 121 ℃ for 15min.
Growth conditions	Temperature: 30°C Atmosphere: Aerobic
Storage conditions	-20 ℃
Sharing mode	Public welfare sharing

Bacillus subtilis

No.:353859 / ATCC 6633

Product format: quantitative bacteria tablets

Number of viable bacteria : 0.5~1.0×10 4CFU/bottle

Product batch: 191227

Valid until : 2020.12.26

Date of inspection: 2019.12.29

Sampling ratio : ≥2%

Report number : RS19353859-1

Biosafety level : 1 , handle in ultra-clean table or safety cabinet

item	description	test result
stain microscopy	Gram-positive, observe cell morphology under microscope	G + cocci
colony morphology	observation of single colony morphology	Gray, dry, flat, irregular edges
purity	non-selective medium culture, colony observation and microscopic examination	No bacteria
Reference value of live bacteria	non-selective medium plate, live bacteria count	9040CFU/bottle
phenotype and genotype analysis	phenotypic testing, gene sequencing	qualified
test conclusion	after quality inspection by BNCC quality control testing system, the product is qualified

Product list :One bottle of 0.5~1.0×10 4CFU quantitative strain, one bottle of 1mL dissolved solution and one copy of strain certificate;

Validity period: unopened, below -20 ℃, 12 months;

Handling procedure:

1 Open the penicillin-bottle according to the arrow indication on the vial cap;
2 Transfer the strain tablets into the vial of solution, capped with rubber plug, and shake to dissolve
3 It will be 0.5~1.0x10 4 cfu/ml after full dissolved, and use appropriate amount according to the operation demand
4 The bacterial tablets shall be used up once and shall not be retained once the penicillin bottled is opened.

Notes:

1 This product is a quantitative strain. Improper storage conditions and handling procedure will affect the accuracy of the strain quantities. Therefore, please operate in strict accordance with the above mentioned instructions.
2 Waste generated from the handling process should be discarded after high-pressure sterilization

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