Neisseria gray|BNCC359185 |BNCC

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Neisseria cinerea

Culture medium	Columbia agar chocolate plate (CA-C): casein pancreatin digest 10.0g, cardiopancreatin digest 3.0g, corn starch 1.0g, meat gastric enzyme digest 5.0g, yeast leaching powder 5.0g, sodium chloride 5.0, agar 20.0g, distilled water 1.0L,pH 7.3±0.2. After sterilization at 121 ℃ for 15min, cool to 80-90 ℃, then add 5% sterile defibrated sheep blood, shake well and pour the plate to cool for later use.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	37 ℃;24-48h;5% CO2;
Storage conditions	2-8 ℃
morphology	Size: 1-2mm Shape: Round Edge: Neat Transparency: Opaque Color: off-white bulge: middle bulge surface: bright and smooth texture: moist and easy to stir up
Separation substrate	Nasopharynx
Sharing mode	Public welfare sharing

Neisseria cinerea

Storage conditions: 2~8 ℃

No. 359185

Product format: freeze dried,200ul

Validity period: 6 years

Biosafety level: 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar plate is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:37°C, 5% CO2, chocolate tablet (ready-to-use), 18-24h. Item test results Rejuvenation: Resurrectable, 24h, the flat bacterial layer is obvious, the typical colony morphology: (as shown in the above picture) Size: 1-2mm Shape: Round Edge: neat Transparency: Opaque Color: off-white Prominence: Middle convex surface : Bright and smooth Texture: Moist and easy to provoke Conclusion: Good rejuvenation, no abnormal colony morphology, qualified。

Recovery steps:

①Prepare 1-2 of above mentioned plates;

②Open in the the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;

③Draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;

④Put the plates under the above culture conditions for cultivation 18-24h，and the strain can be used when it grows out.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability:	good viability，in 24 hours,obvious strain layer occurs on the plate; colony is typical
colony morphology: (pictured above)	Size: 1-2mm Shape: Round Edge: Neat Transparency: Opaque Color: off-white Protuberance: Middle Protuberance Surface: Bright and Smooth Texture: Wet and Easy to Stir
conclusion: good viability, no abnormal colony morphology, qualified;

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