Pleurotus ostreatus|BNCC358967 |BNCC

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Pleurotus ostreatus

Culture medium	Comprehensive PDA agar (CPDA): potato boil 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4 · 7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.
Subculture procedure	(1) Prepare 1-2 pieces of the plate; (2) sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet; (3) cut into the pieces with aseptic inoculation hoe and inoculation shovel (see attached page). the size of square pieces is 0.5 × 0.5cm2; (4) lay flat the small pieces to the center of the agar plate; (5)put the plates under the above culture conditions, and the strains can be used when they grow.
Growth conditions	28 ℃;5-7 days; Aerobic;
Storage conditions	2-8 ℃
morphology	Small filamentous fungi, the colonies are obvious on the integrated PDA medium, white, dense and vigorous hyphae, spreading to the edge of the plate, and the back of the medium is light yellow.
Sharing mode	Public welfare sharing

Pleurotus ostreatus

Storage conditions: 2~8 ℃

No. 358967

Product format: agar slant in 14mm test tube

Validity period: growing culture, in 30 days

Biosafety level: 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The agar slant can be used directly. In principle, it can be used many times without contamination within the validity period, but viability will gradually decrease with time. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Culture conditions:28 ℃, aerobic, integrated PDA,5-7 days. Comprehensive PDA: potato boiling solution 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4·7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling solution: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect filtrate to a constant volume of 1.0L.

Recovery steps:

① Prepare 1-2 pieces of PDA plates;

②Sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet;

③Cut into the pieces with aseptic inoculation hoe and inoculation shovel (see attached page). the size of square pieces is 0.5 × 0.5cm²;

④ Lay flat the small pieces to the center of the agar plate;

⑤ Put the plates under the above culture conditions, and the strains can be used when they grow.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	Test results
Viability	good viability. in 48-72 hours，obvious strain layer occurs on the plate
Colony morphology:	Small filamentous fungi, the colonies are obvious on the integrated PDA medium, white, dense and vigorous hyphae, spreading and growing to the edge of the plate, and the back of the medium is light yellow.
Conclusion:	good viability, no abnormal colony morphology, qualified