BeNa Culture Collection

Novosphingobium gossypii-BNCC
  • BNCC
  • Novosphingobium gossypii-BNCC

Novosphingobium gossypii

  • Price: $ 172
  • number:BNCC341740
  • Form:
    Size: 1-2mm Shape: Round Edge: Neat Transparency: Opaque Color: Orange Protuberance: Middle convex Surface: Bright and Smooth Texture: Wet and Easy to Stir
Standard strain Quantitative strain DNA extraction
Package:
  • freeze dried
    Freeze-dried: lyophilized strain, freeze dried vial, used up at one time and not be retained
  • agar slant
    Agar slant: growing culture, ready to use, Short term preservation, convenient access
Essential Information Certificate Related Products
Novosphingobium gossypii
Culture medium Tryptone soybean medium (TSA/TSB): tryptone 17.0g, soybean peptone 3.0g, sodium chloride 5.0g, dipotassium hydrogen phosphate 2.5g, glucose 2.5g, agar 20.0g (without liquid medium), distilled water 1.0L,pH 7.3±0.2. Sterilization at 121 ℃ for 15min.
Subculture procedure (1) Prepare a test tube containing 5~10mL of liquid  medium and 2 plates; (2) open it in the biosafety cabinet, heat the tip of ampoule in the flame, quickly drop sterile water to creak it, and break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions 30 ℃;18-24h; Aerobic;
Storage conditions 2-8 ℃
morphology Size: 1-2mm Shape: Round Edge: Neat Transparency: Opaque Color: Orange Protuberance: Middle convex Surface: Bright and Smooth Texture: Wet and Easy to Stir
application Taxonomic research.
Sharing mode Public welfare sharing

Corynebacterium glutamicum

Description 

1. BNCC No.: BNCC 185409

2. Biosafety level: 4

Storage conditions:

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

Growth Conditions 

nutrient gravy agar: beef paste 3.0g, peptone 10.0g, naCl 5.0g, agar 15.0g, distilled water 1.0L,pH7.0. [note] adding 5mg MnSO·H2 when culturing Bacillus; it is  is beneficial to spore production. pH7.0. 121 ℃,15min.

2. Atmosphere: aerobic

3. Temperature:  30 ℃

Notes: 
1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization

Download certificate
Please set your password: