Trichoderma virens|BNCC339869 |BNCC

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Trichoderma virens

Culture medium	CDA: 3g of sodium nitrate, 1g of dipotassium hydrogen phosphate, 0.5g of magnesium sulfate, 0.5g of potassium chloride, 0.01g of ferrous sulfate, 30g of sucrose, 15g of agar, 1.0L of distilled water and 6.2±0.2 of pH. Sterilization at 121 ℃ for 15min.
Subculture procedure	(1) Prepare 1-2 pieces of the plate; (2) sterilize the surface of the plate and open it in biosafety cabinet; (3) Absorb 1-3ml of sterile water into the plate, scrape the bacterial lawn back and forth on the surface of the plate with a sterile spreader to make a bacterial suspension; (4) suck the bacteria suspension with a pasteur pipette, dispense it into a fresh plate evenly. (5) put the plates under the growth conditions.
Growth conditions	28 ℃;5-7 days; Aerobic
Storage conditions	2-8 ℃
morphology	Small filamentous fungi, with obvious colonies on Cha's agar medium, the plate layer hyphae are white villi in the early stage, and gradually turn yellow, and produce yellow-green spores in the later stage, and the back of the medium is light yellow.
application	Degradable plastics, antifungal tests Degradable plastics, antifungal tests, environmental tests for electronic and electrical products-part 2: experimental methods, tests j and guidelines: mildew growth (GB/T 2423.16-2008)
Sharing mode	Public welfare sharing

Description

1. Name: Trichoderma virens

2. BNCC No.: 339869

3. Biosafety level:4

Storage conditions:

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

Growth Conditions

1, Czapek ' Sagar: sucrose 30.0g,NaNO3 3.0 g,MgSO4·7H2O 0.5 g,KCl 0.5 g,FeSO4·4H2O 0.01 g,K2HPO4 1.0 g, agar 15.0g, distilled water 1.0 L,pH 6.0-6.5, 115 ℃,20min.

2.Atmosphere:aerobic

3. Temperature: 25 ℃

Notes:

1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization