Trichoderma reesei|BNCC337997 |BNCC

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Trichoderma reesei

Culture medium	Comprehensive PDA agar (CPDA): potato boil 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4 · 7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.
Subculture procedure	(1) Prepare 1-2 pieces of the plate; (2) sterilize the surface of the plate and open it in biosafety cabinet; (3) Absorb 1-3ml of sterile water into the plate, scrape the bacterial lawn back and forth on the surface of the plate with a sterile spreader to make a bacterial suspension; (4) suck the bacteria suspension with a pasteur pipette, dispense it into a fresh plate evenly. (5) put the plates under the growth conditions.
Growth conditions	28 ℃;3-5 days; Aerobic
Storage conditions	2-8 ℃
morphology	Small filamentous fungi, with obvious colonies on integrated PDA medium, white hyphae at the initial stage, dense and low flat, and yellow spores overgrown plates later.
application	Decompose cellulose
Sharing mode	Public welfare sharing

1. Description

(1)Name:Trichoderma reesei

(2)BNCC No.: 337997
(3)Biosafety level: 4
2. Storage conditions
Storage of freezed dried ampoule and agar slant at 2°C to 8°C.
3. Growth Conditions
(1)Comprehensive PDA: potato boiled juice 1000mL, potassium dihydrogen phosphate 3g, magnesium sulfate 1.5g, glucose 20g, vitamin B1 10mg, agar 20g,pH natural. Potato boiling solution: 200g of potato, peeled, cut into pieces, boiled in distilled water for 30min, constant volume of filtrate to 1000mL for later use. 121 ℃,15min.
(2) Atmosphere: aerobic
(3)Temperature: 25 ℃
4. Notes:

(1)Normal culturing time, 1-2days for bacterial, 3days for yeast, 5-7days for mould, 7-10days for fungal.

(2)Agar slant shall be inoculated asap, and long-term storage is not recommended.

(3)Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

(4)Users should ensure the safe storage and operation of the strain.Waste generated from the handling process should be discarded after high-pressure sterilization