Rhizomucor miehei|BNCC337563 |BNCC

Rhizomucor miehei

Price： $ 115
number：BNCC337563
Form：
Small filamentous fungi have obvious colonies on the comprehensive PDA medium. The hyphae of the plate layer are white in the early stage and gradually turn gray. They spread and grow to cover the whole plate. They produce gray spores in the later stage and the back of the medium is light yellow.

Standard strain Quantitative strain DNA extraction

Package：

freeze dried
Freeze-dried: lyophilized strain, freeze dried vial, used up at one time and not be retained
agar slant
Agar slant: growing culture, ready to use, Short term preservation, convenient access

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Essential Information Certificate Related Products

Rhizomucor miehei

Culture medium	Comprehensive PDA agar (CPDA): potato boil 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4 · 7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.
Subculture procedure	(1) Prepare 1-2 pieces of the plate; (2) sterilize the surface of the plate and open it in biosafety cabinet; (3) Absorb 1-3ml of sterile water into the plate, scrape the bacterial lawn back and forth on the surface of the plate with a sterile spreader to make a bacterial suspension; (4) suck the bacteria suspension with a pasteur pipette, dispense it into a fresh plate evenly. (5) put the plates under the growth conditions.
Growth conditions	37 ℃;5-7 days; Aerobic
Storage conditions	2-8 ℃
morphology	Small filamentous fungi have obvious colonies on the comprehensive PDA medium. The hyphae of the plate layer are white in the early stage and gradually turn gray. They spread and grow to cover the whole plate. They produce gray spores in the later stage and the back of the medium is light yellow.
application	Chymosin production
Sharing mode	Public welfare sharing

1.Description

1. Name: Rhizomucormiehei

2. BNCC No.:337563

3. Biosafety level: 4

2. Storage conditions

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3. Growth Conditions

1. CPDA medium: potato boiling solution 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4·7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L. Sterilization at 121 ℃ for 15min.

2. Atmosphere: aerobic

3. Temperature: 37 ℃.

4.Notes:

1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization.