Streptomyces antibioticus (Waksman et Woodruff) Waksman et Henrici|BNCC336593 |BNCC

Streptomyces antibioticus (Waksman et Woodruff) Waksman et Henrici

Price： $ 86
number：BNCC336593
Form：
Size: 1-2mm Shape: Round Edge: Irregular Transparency: Opaque Color: milky white bulge: middle bulge surface: bright texture: dry

Standard strain Quantitative strain DNA extraction

Package：

freeze dried
Freeze-dried: lyophilized strain, freeze dried vial, used up at one time and not be retained
agar slant
Agar slant: growing culture, ready to use, Short term preservation, convenient access

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Essential Information Certificate Related Products

Streptomyces antibioticus (Waksman et Woodruff) Waksman et Henrici

Culture medium	Streptomyces medium No. 2 (ISP-2): yeast powder 4.0g, malt powder 10.0g, glucose 4.0g, agar 20.0g,pH 7.2±0.2. Sterilization at 121 ℃ for 15min.
Subculture procedure	(1) Prepare 1-2 pieces of the plate; (2) sterilize the surface of the plate and open it in biosafety cabinet; (3) Absorb 1-3ml of sterile water into the plate, scrape the bacterial lawn back and forth on the surface of the plate with a sterile spreader to make a bacterial suspension; (4) suck the bacteria suspension with a pasteur pipette, dispense it into a fresh plate evenly. (5) put the plates under the growth conditions.
Growth conditions	28 ℃;2-4 days; Aerobic
Storage conditions	2-8 ℃
morphology	Size: 1-2mm Shape: Round Edge: Irregular Transparency: Opaque Color: milky white bulge: middle bulge surface: bright texture: dry
application	Actinomycin A and B are produced.
Sharing mode	Public welfare sharing

Streptomyces antibioticus (Waksman et Woodruff) Waksman et Henrici

Description

1. BNCC No.:336593

2. Biosafety level: 4

Storage conditions:

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

Growth Conditions

1. ISP-2 medium: yeast powder 4.0g, malt powder 10.0g, glucose 4.0g, agar 20.0g, distilled water 1.0 L,pH7.2±0.2. 121 ℃,15min.

2. Atmosphere:aerobic

3. Temperature: 28-30 ℃,5-7 days

Notes:

1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization.