Trichoderma viride|BNCC336558 |BNCC

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Trichoderma viride

Culture medium	Comprehensive PDA agar (CPDA): potato boil 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4 · 7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	28 ℃;5-7 days; Aerobic;
Storage conditions	2-8 ℃
morphology	The morphology of the integrated PDA agar medium was obvious. The colony was white cotton wool at the beginning, and green spores were produced later, dry, folded, and the reverse side of the colony was light yellowish brown, spreading on the surface of the plate.
application	Cellulase production.
Sharing mode	Public welfare sharing

Trichoderma viride

storage conditions: 2~8 ℃

number: 336558

product format: freeze dried, 200ul

shelf life: 24 months

Safety level: 1, handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Culture conditions:25-28 ℃, aerobic, integrated PDA. Comprehensive PDA:20% potato juice 1L, glucose 20g ,KH₂PO₄ 3g, MgSO₄.7H₂O 1.5g, thiamine trace, agar 15g,pH natural.

Recovery steps:

(1)Prepare 2 pieces of PDA plates or 2 agar slants;

(2)Open the ampoule in the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3)draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate; or transfer appropriate solution to

the agar slant.

(4)place the plates uprightly and agar slant obliquely under the above culture conditions for 5-7 days. Or contact our technicians for the inoculation of agar slant - filamentous fungi.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test result
Viability:	good viability,in 5 days, plate colony is obvious
colony morphology:	The morphology of the bacteria is obviously visible in the integrated PDA agar medium, and the colony is white cotton wool at the initial stage, Later, green spores are produced, dry, wrinkled, and the reverse side of the colony is light yellowish brown, spreading on the surface of the flat plate.
Conclusion:	good viability, no abnormal colony morphology, completely consistent with the following figure, qualified