Streptomyces splendens (Yan et Deng) Yan et al.|BNCC335934 |BNCC

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Streptomyces splendens (Yan et Deng) Yan et al.

Culture medium	Gauche Synthesis No. 1 Agar (GM): 20g of soluble starch, 0.5g of sodium chloride, 0.01g of ferrous sulfate, 1.0g of potassium nitrate, 0.5g of dipotassium hydrogen phosphate, 0.5g of magnesium sulfate, 15g of agar, 1.0L of distilled water and 7.3±0.2 of pH. Sterilization at 121 ℃ for 15min.
Growth conditions	28 ℃, aerobic
Storage conditions	2~8 ℃
Sharing mode	Public welfare sharing

1.Description

1. Name: Streptomyces splendens (Yan et Deng) Yan et al

2. BNCC No.:335934

3. Biosafety level: 4

2. Storage conditions:

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3. Growth Conditions

1. Gao's synthetic agar No. 1: KNO3 1g, soluble starch 20g,K2HPO4 0.5g, MgSO4.7H2O 0.5g,NaCl 0.5g,FeSO4 0.01g, agar 20g, water 1000ml,pH 7.2-7.4

2. Atmosphere: aerobic

3. Temperature: 28 ℃

4. Notes:
1.Normal culturing time, 1-2days for bacterial, 3days for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and long-term storage is not recommended.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Users should ensure the safe storage and operation of the strain.Waste generated from the handling process should be discarded after high-pressure sterilization