BeNa Culture Collection

Burkholderia multivorans Vandamme et al.-BNCC
  • BNCC
  • Burkholderia multivorans Vandamme et al.-BNCC

Burkholderia multivorans Vandamme et al.

  • Price: $ 458
  • number:BNCC301368
  • Form:
    Size: 0.5-1mm Shape: round Edge: neat Transparency: Opacity Color: light yellow Protuberance: Middle bulge Surface: bright and smooth Texture: moist and easy to stir up
Standard strain Quantitative strain DNA extraction
Package:
  • freeze dried
    Freeze-dried: lyophilized strain, freeze dried vial, used up at one time and not be retained
  • agar slant
    Agar slant: growing culture, ready to use, Short term preservation, convenient access
Essential Information Certificate Related Products
Burkholderia multivorans Vandamme et al.
Culture medium Tryptone soybean medium (TSA/TSB): tryptone 17.0g, soybean peptone 3.0g, sodium chloride 5.0g, dipotassium hydrogen phosphate 2.5g, glucose 2.5g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.3±0.2. Sterilization at 121 ℃ for 15min.
Subculture procedure (1) According to the culture conditions, the bacteria are first cultured into a liquid suspension; ② Measure the absorbance of the bacterial suspension and calculate the initial concentration of the bacterial suspension according to the standard formula; (3) The initial concentration multiplied by the survival rate is the actual concentration after freeze-drying; (4) Dilute the bacterial suspension according to the actual concentration after freeze-drying to obtain the target concentration, and then subpack and freeze-dry;
Growth conditions 30 ℃;18-24h; Aerobic;
Storage conditions 2-8 ℃
morphology Size: 0.5-1mm Shape: round Edge: neat Transparency: Opacity Color: light yellow Protuberance: Middle bulge Surface: bright and smooth Texture: moist and easy to stir up
Separation substrate Clinical specimen - human, Cystic fibrosis patient, Belgium, 1992
Sharing mode Public welfare sharing

Burkholderia multivorans

Storage conditions: 2~8 ℃

No. 301368

Product format: freeze dried,200ul

Validity period: 6 years

Biosafety  level:  2, handle in safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions: 30℃, aerobic, tryptone soybean medium, 18-24h. Tryptone soybean medium: tryptone 17.0g, soy 3.0g, sodium chloride 5.0g, dipotassium hydrogen phosphate 2.5g, glucose 2.5g, agar 20.0g (not included in liquid medium), distilled water 1.0L, pH 7.3 ±0.2. Sterilize at 121℃ for 15min.

Recovery steps:

(1) Prepare a test tube containing 5-10mL of liquid culture medium and 2 plates; 

(2) Open it in the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; 

(3) Draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; 

(4) Inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; 

(5) Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.

Recovery record:  According to the recovery instructions, the results of the recovery are reported as follows:

                                                       

Item test results
viability

good viability,in 18h, plate bacteria layer obvious;

colony is typical on the streaked plate

colony morphology: (above)

size: 0.5-1mm shape: round edge: neat transparency: opaque

color: light yellow uplift: middle convex surface: bright and smooth texture: moist and easy to stir

Conclusion good viability, no abnormal colony morphology, qualified
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