Simplicillium lamellicola|BNCC231351 |BNCC

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Simplicillium lamellicola

Culture medium	Comprehensive PDA agar (CPDA): potato boil 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4 · 7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.
Subculture procedure	(1) Prepare 1-2 pieces of PDA plate; (2) sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet; (3) cut into the pieces with aseptic inoculation hoe and inoculation shovel (see attached page). the size of square pieces is 0.5 × 0.5cm2; (4) lay flat the small pieces to the center of the agar plate; (5)put the plates under the above culture conditions, and the strains can be used when they grow.
Growth conditions	28 ℃,5-7 days, aerobic
Growth characteristics	The fruiting body has the habit of light-stalked mushrooms or small mushrooms to money bacteria, the cap is small to large, with a distinctly pointed umbilical process, with set-up stripes, there are fungus curtain residues, the surface is dry to adhesive, the stalk is raw, Solid, the stalk base has a long vacation root extending underground
Storage conditions	2-8 ℃
morphology	Small filamentous fungi, with obvious colonies on comprehensive PDA medium, white hyphae, dense and low flat, striped on the surface, and flat Spread and grow concentric circles at the edge of the plate.
application	Research; Production
Sharing mode	Public welfare sharing

Termitomyces albuminosus

No. :231351

Storage conditions: 2~8 ℃

Product format: agar slant in 14mm test tube

Validity period: growing culture, in 30 dyas

Biosafety level: 1, handle in ultra-clean table or safety cabinet

Receiving notice: If any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:25-28 ℃, aerobic, integrated PDA,5-7 days. Comprehensive PDA: potato boil 1.0L, glucose 20.0g,KH₂PO₄ 3.0g,MgSO₄·7H₂O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.

Recovery steps:
(1)Prepare 1-2 pieces of PDA plates;
(2)Sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet;
(3) Cut into the pieces with aseptic inoculation hoe and inoculation shovel (see attached page). the size of square pieces is 0.5 × 0.5cm2;
(4) Lay flat the small pieces to the center of the agar plate;
(5)Put the plates under the above culture conditions, and the strains can be used when they grow.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test result
viability:	good viability， in 5-7 days plate colony is obvious
colony morphology:	small filamentous fungi with obvious colonies on integrated PDA medium, hyphae are white, dense and flat, with stripes on the surface, spreading and growing to concentric circles on the edge of the plate.
conclusion:	good viability, no abnormal colony morphology, qualified