Fusarium oxysporum f. sp. cucumerinum|BNCC227992 |BNCC

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Fusarium oxysporum f. sp. cucumerinum

Culture medium	Potato Glucose Agar (PDA): Potato Boiled Solution 1.0L, Glucose 20.0g, Agar 15.0g, Natural pH. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.
Subculture procedure	(1) Prepare 1-2 pieces of PDA plate; (2) sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet; (3) cut into the pieces with aseptic inoculation hoe and inoculation shovel (see attached page). the size of square pieces is 0.5 × 0.5cm2; (4) lay flat the small pieces to the center of the agar plate; (5)put the plates under the above culture conditions, and the strains can be used when they grow.
Growth conditions	28 ℃,5-7 days, aerobic
Storage conditions	2-8 ℃
morphology	Small filamentous fungi, with obvious colonies on PDA medium, purple-red hyphae, dense and vigorous, spreading to the edge of the plate, The back of the medium is brownish yellow.
application	Classification; Research; Cucumber Fusarium oxysporum
Sharing mode	Public welfare sharing

1.Description

1. Name：Fusarium, oxysporum f. sp. cucumerinum

2. BNCC No.：227992

3. Biosafety level：4

2.Storage conditions：

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3.Growth Conditions

1, PDA medium: potato extract 1.0 L, glucose 20.0g, agar 15.0g, natural pH.

2. Atmosphere：aerobic

3. Temperature：28 ℃

4.Notes：

1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization