Bacillus lichenformis|BNCC221464 |BNCC

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Bacillus lichenformis

Culture medium	Nutritional gravy medium (NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mg MnSO4 · H2O when culturing Bacillus. it is beneficial to produce spores.
Growth conditions	30 ℃,18-24h, aerobic
Storage conditions	2-8 ℃
Separation substrate	Long-term deciduous humus layer
application	Cellulose degrading bacteria
Sharing mode	Public welfare sharing

1.Description

1. Name：Bacillus lichenformis

2. BNCC No.：221464

3. Biosafety level：4

2.Storage conditions：

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3.Growth Conditions

1. Nutritious gravy agar: beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 15.0g, distilled water 1.0L,pH7.0. [Note] Add5mg MnSO4·H2Owhen culturing Bacillus，which is beneficial to spore production. pH7.0. 121 ℃,15min.

2. Atmosphere：aerobic

3. Temperature：30 ℃

4.Notes：

1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization