BeNa Culture Collection

Bacillus lichenformis-BNCC
  • BNCC
  • Bacillus lichenformis-BNCC

Bacillus lichenformis

  • Price: $ 172
  • number:BNCC221464
Standard strain Quantitative strain DNA extraction
Package:
  • freeze dried
    Freeze-dried: lyophilized strain, freeze dried vial, used up at one time and not be retained
  • agar slant
    Agar slant: growing culture, ready to use, Short term preservation, convenient access
Essential Information Certificate Related Products
Bacillus lichenformis
Culture medium Nutritional gravy medium (NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mg MnSO4 · H2O when culturing Bacillus. it is beneficial to produce spores.
Growth conditions 30 ℃,18-24h, aerobic
Storage conditions 2-8 ℃
Separation substrate Long-term deciduous humus layer
application Cellulose degrading bacteria
Sharing mode Public welfare sharing

1.Description 

1. Name:Bacillus lichenformis

2. BNCC No.:221464

3. Biosafety level:4

2.Storage conditions:

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3.Growth Conditions 

1. Nutritious gravy agar: beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 15.0g, distilled water 1.0L,pH7.0. [Note] Add5mg MnSO4·H2Owhen culturing Bacillus,which  is beneficial to spore production. pH7.0. 121 ℃,15min.

2. Atmosphere:aerobic

3. Temperature:30 ℃

4.Notes:

1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization

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