Rotavirus A RNA lab quality control|BNCC367892 |BNCC

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Rotavirus A RNA lab quality control

Culture medium	Special medium for VR-2018: EMEM +2 μg/mL Trypsin
Subculture procedure	Remove from the refrigerator, melt completely, vortex and mix well, ready to use. Note: The quality control is inactivated particles, which need to be used after nucleic acid extraction. The extraction or purification reagents should be prepared by themselves, and repeated freezing and thawing should be avoided before extraction. The minimum sampling volume is 200 μl.
Growth conditions	The culture temperature is 37 ℃;1-7 days of culture time; Gas environment 5% CO2 + 95% air;
Storage conditions	-80 ℃
Safety level	2
Separation substrate	The strain was isolated from the diarrhoeal faeces of rotavirus-positive patients and deposited by the National Institute of Allergy and Infectious Diseases.
application	The quality control is developed by Henan engineering center of industrial microbial strain, which is rotavirus A inactivated suspension, which can be used for method establishment, working standard assignment, method confirmation and evaluation, product quality control, etc. by digital PCR value.
Sharing mode	Public welfare sharing