Reference substance for total number of colonies in food|BNCC359084 |BNCC

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Reference substance for total number of colonies in food

Culture medium	Nutritional gravy medium (NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (without liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculture procedure	Before use, take it out from -20 ℃ and balance it at room temperature for 10 minutes, and accurately weigh (1.09±0.01)g(20 ℃) of sterile distilled water to re-dissolve the freeze-dried standard substance with a balance.
Growth conditions	37 ℃;18-24 hours; Aerobic;
Storage conditions	-20 ℃, kept away from light
Safety level	2
application	This standard substance has a wide range of uses in microbial laboratory capability verification, microbial measurement method evaluation and instrument calibration, and microbial medium growth rate evaluation, and provides metrological traceability guarantee for food safety and microbial testing.
Sharing mode	Public welfare sharing