Beauveria bassiana|BNCC192026 |BNCC

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Beauveria bassiana

Culture medium	Comprehensive PDA agar (CPDA): potato boil 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4 · 7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	28 ℃;3-7 days; Aerobic
Growth characteristics	The conidia stems are solitary, irregularly gathered or arranged in a spoke shape, single-celled colorless, born on short and small stalks.
Storage conditions	2-8 ℃
morphology	Small filamentous fungi have obvious colonies on the comprehensive PDA medium, with white and dense hyphae, raised on the surface of the colony, striped, spreading to the edge of the plate, and growing on the back of the medium with light yellow and radioactive folds.
application	Research. Insect control.
Sharing mode	Public welfare sharing

Beauveria bassiana

No.: 192026

Storage conditions: 2~8 ℃

Product format: freeze dried,200ul

Validity period: 6 years

Biosafety level: 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:28 ℃, aerobic, 3-7 days, comprehensive PDA agar: potato boiling solution 1.0L, glucose 20.0g,KH₂PO₄ 3.0g,MgSO₄.7H₂O 1.5g, vitamin B1 trace, agar 20.0g (not included in liquid medium),pH 6.0 ±0.2. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.

Recovery steps:

(1)Prepare a test tube containing 5-10mL of liquid culture medium and 2 plates;

(2) Open it in the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3) Draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly;

(4) Inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates;

(5) Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test results
Viability:	good viability,in 5 days plate colony obvious
colony morphology: (above)	small filamentous fungi with obvious colonies, white and dense hyphae and raised colony surface on integrated PDA culture medium, striped, spreading to the edge of the plate, light yellow on the back of the medium, with radioactive folds.
conclusion:	good viability, no abnormal colony morphology, qualified