BeNa Culture Collection

Neurospora sitoplila-BNCC
Neurospora sitoplila-BNCC
Neurospora sitoplila-BNCC
  • BNCC
  • Neurospora sitoplila-BNCC
  • Neurospora sitoplila-BNCC
  • Neurospora sitoplila-BNCC

Neurospora sitoplila

  • Price: $ 143
  • number:BNCC189192
  • Form:
    Small filamentous fungi, with obvious colonies on integrated PDA medium, pink, pink spores and full of plates.
Standard strain Quantitative strain DNA extraction
Package:
  • freeze dried
    Freeze-dried: lyophilized strain, freeze dried vial, used up at one time and not be retained
Essential Information Certificate Related Products
Neurospora sitoplila
Culture medium Comprehensive PDA agar (CPDA): potato boil 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4 · 7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.
Subculture procedure (1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions 28 ℃;2-3 days; Aerobic
Storage conditions 2-8 ℃
morphology Small filamentous fungi, with obvious colonies on integrated PDA medium, pink, pink spores and full of plates.
Sharing mode Public welfare sharing

Neurospora sitoplila

Storage conditions: 2~8 ℃

No. 189192

Product format: freeze dried,200ul

Validity period: 6 years

Biosafety  level:  1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions: 28℃, aerobic, comprehensive PDA, 2-3 days, comprehensive PDA: potato cooking liquid 1.0L, glucose 20.0g, KH2PO4 3.0g, MgSO4 7H2O 1.5g, vitamin B1 trace, agar 20.0g, pH6.0±0.2 .121℃, 15min sterilization. Potato cooking liquid: Weigh 200g peeled potato pieces, boil in boiling water for 30min, collect the filtrate and dilute to 1.0L.

Recovery steps:

(1) Prepare 1-2 of above mentioned plates; 

(2) Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps; 

(3) Draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate; 

(4) Put the plates under the above culture conditions for cultivation for 2-3 days.

Recovery record:  According to the recovery instructions, the results of the recovery are reported as follows:
                                                                              

Item test results
viability good viability, in 2-3 days plate colony is obvious
colony morphology: (above) Small filamentous fungi with obvious colonies on integrated PDA medium, producing pink spores and growing plates.
Conclusion good viability, no abnormal colony morphology, qualified

 

Please set your password: