Pseudomonas aeruginosa|BNCC185967 |BNCC

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Pseudomonas aeruginosa

Culture medium	Nutritional gravy medium (NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mg MnSO4 · H2O when culturing Bacillus. it is beneficial to produce spores.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	37 ℃;18 -- 24h; Aerobic;
Growth characteristics	G-bacilli, lysine decarboxylase, ornithine decarboxylase were negative, oxidase, arginine dihydrolase were positive; can grow at 41 ℃, indole, volt-pu test negative, fermentation of glucose, fructose, no fermentation of lactose, no H2S. Produce pyogenin and fluorescent pigment. Specialized aerobic, optimum pH7.4 ~ 7.6.
Storage conditions	2-8 ℃
morphology	The colony diameter is 1-2mm, round, with neat edges, opaque, yellow on the front, light color, convex in the middle, bright surface, smooth surface, moist texture, easy to pick up, G-(red), bacilli
application	Research; Analytical testing. research, quality control.
Sharing mode	Public welfare sharing

Pseudomonas aeruginosa

Storage conditions: 2~8 ℃

No.: 185967

Product format: freeze dried,200ul

Validity period: Freeze-dried tube for 6 years

Biosafety level: 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Recovery/subculture:37°C, aerobic, nutrient gravy medium. Nutritional agar medium: beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH7.0. 121℃,15min

Recovery steps:

(1)Prepare a flask of liquid media or two agar plates.

(2)Open it in biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3)Draw 0.5mL of liquid medium into the freeze dried ampoule,make the strain pellet fully dissolved. transfer the liquid suspension to the liquid media, and culture in plate shaker at 28℃ for 18-24 hours (140r/min); or directly dispense 200ul of the liquid suspension into a agar plate evenly, then put the plates in incubator at 28℃ for 24-48 hours.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test result
viability	good viability, in20h liquid medium become turbid, obvious strain layer occurs on the plate,colony is typical on marked plate.
colony morphology	size: medium color: off-white late green shape: round edge: irregular wet and dry: wet and smooth: smooth transparency: opaque uplift: uplift
conclusion	good viability, and colony morphology is not abnormal, completely consistent with the above figure, qualified