Aspergillus restrictus|BNCC144254 |BNCC

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Aspergillus restrictus

Culture medium	Concentrated sugar Chashi agar (CDA-2): sucrose 200.0g,NaNO3 3.0g,MgSO4 · 7H2O 0.5g,KCl 0.5g,FeSO4 · 4H2O 0.01g,K2HPO4 1.0g, agar 15.0g, distilled water 1.0L,pH 6.0-6.5. Sterilization at 121 ℃ for 15min.
Subculture procedure	(1) Prepare 1-2 pieces of the plate; (2) sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet; (3) cut into the pieces with aseptic inoculation hoe and inoculation shovel (see attached page). the size of square pieces is 0.5 × 0.5cm2; (4) lay flat the small pieces to the center of the agar plate; (5)put the plates under the above culture conditions, and the strains can be used when they grow.
Growth conditions	28 ℃;7-10 days; Aerobic;
Storage conditions	2-8 ℃
morphology	Small filamentous fungus, with obvious colonies on concentrated sugar Cha's agar medium, the initial hyphae are dark green, dense and vigorous, and later produce gray green Color spores, spread to the edge of the plate and grow, the back of the medium is gray-green.
Separation substrate	Moldy fiber fabric
application	Fibre fabric mold
Sharing mode	Public welfare sharing

Aspergillus restrictus

Storage conditions: 2~8 ℃

No. 144254

Product format: freeze dried, 200ul

Validity : 6 years

Biosafety level: 1 , handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar plate is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:28 ℃, aerobic, concentrated sugar Cha's agar, 7-10 days. Concentrated sugar Chashi agar: sucrose 200.0g,NaNO3 3.0g,MgSO4 ·7H2O 0.5g,KCl 0.5g, FeSO4 ·4H2O 0.01g,K2HPO4 1.0g, agar 15.0g, distilled water 1.0L, pH 6.0-6.5. Sterilization at 121 ℃ for 15min.

Recovery steps:
(1) Prepare 1-2 of above mentioned plates;
(2) Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;
(3) draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;
(4) Put the plates under the above culture conditions for cultivation for 7-10 days.
Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability:	good viability, in 7 days strain layer become obvious
colony morphology:	small filamentous fungi, with obvious colonies on concentrated sugar cha's agar medium, the initial hyphae are dark green, dense and vigorous, and then produce gray-green spores, which spread to the edge of the plate and grow, and the back of the medium is gray-green.
Conclusion:	good viability, no abnormal colony morphology, qualified