BeNa Culture Collection
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Freeze-dried: lyophilized strain, freeze dried vial, used up at one time and not be retained
| Culture medium | 2216E seawater agar (English name: 2216E): peptone 5.0g, yeast extract 1.0g, ferric citrate 0.1g, potassium bromide 0.08g,NaCl 19.45g,MgCl2 8.8g,Na2SO4 3.24g,CaCl2 1.8g,KCl 0.55g,NaHCO3 0.16g,NH4NO3 0.0016g,Na2HPO4 0.008g, strontium chloride 0.034g, boric acid 0.022g, sodium silicate 0.004g, pH 7.4-7.8. Sterilization at 121 ℃ for 15min. |
| Subculture procedure | (1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow. |
| Growth conditions | 30 ℃;24-48h; Aerobic |
| Storage conditions | 2-8 ℃ |
| morphology | The colony diameter is 1-2mm, round, with neat edges, opaque, orange on the front, dark color, convex in the middle, smooth and bright surface, moist texture, easy to stir up, no pigment, G-(red), bacilli, purity: pure |
| Separation substrate | bottom mud |
| Sharing mode | Public welfare sharing |
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