BeNa Culture Collection

Enterococcus chicken-BNCC
  • BNCC
  • Enterococcus chicken-BNCC

Enterococcus chicken

  • Price: $ 172
  • number:BNCC139341
  • Form:
    The colony diameter is 0.5-1mm, round, with neat edges, opaque, gray-white on the front, convex in the middle, gray and smooth surface, easy to pick up, G + (blue-purple), cocci
Standard strain Quantitative strain DNA extraction
Package:
  • freeze dried
    Freeze-dried: lyophilized strain, freeze dried vial, used up at one time and not be retained
Essential Information Related Products
Enterococcus chicken
Culture medium MRS medium (MRS): peptone 10.0g, beef paste 10.0g, yeast powder 4.0g, glucose 20.0g, magnesium sulfate 0.2g, sodium acetate 5.0g, triammonium citrate 2.0g, dipotassium hydrogen phosphate 2.0g, manganese sulfate 0.04g, Tween 80 1.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L. pH 5.7±0.2. Sterilization at 121 ℃ for 15min.
Subculture procedure (1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions 37 ℃;24-48h; Micro aerobic (3%-5% O2,10% CO2);
Storage conditions 2-8 ℃
morphology The colony diameter is 0.5-1mm, round, with neat edges, opaque, gray-white on the front, convex in the middle, gray and smooth surface, easy to pick up, G + (blue-purple), cocci
Separation substrate chicken intestine
application Type strain
Sharing mode Public welfare sharing
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