BeNa Culture Collection
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| Culture medium | Peptone: 10.0, beef extract powder: 8.0, yeast powder: 4.0, glucose: 20.0, magnesium sulfate: 0.2, sodium acetate: 5.0, diammonium hydrogen citrate: 2.0, dipotassium hydrogen phosphate: 2.0, manganese sulfate: 0.04, Tween 80:1.0, agar: 14.0,pH:5.7±0.2(25 ℃) |
| Subculture procedure | ① According to the culture conditions, the bacteria are first cultured into a bacterial suspension; ② Measure the absorbance of the bacterial suspension and calculate the initial concentration of the bacterial suspension according to the standard formula; The initial concentration multiplied by the survival rate is the actual concentration after freeze-drying; ④ According to the actual concentration after freeze-drying, the bacterial suspension was diluted to obtain the target concentration, and then subpacked and freeze-dried; |
| Growth conditions | 37 ℃;48-72h; anaerobic; |
| Growth characteristics | The strain was cultured on nutrient meat juice agar medium at 37 ℃ for 3 days, and the thallus was rod-shaped, 0.61 μm × 1.22~2.13 μm, gram-negative, and did not produce spores. |
| Storage conditions | -20 ℃ |
| Safety level | 1 |
| morphology | Spreading growth, gray spores, gray front, light color, purity: pure |
| Separation substrate | starter culture |
| Sharing mode | Public welfare sharing |
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