BeNa Culture Collection
Culture medium | Yeast extract: 3.0, malt extract: 3.0, glucose: 10.0, casein peptone: 5.0, agar: 20.0,pH:6.2±0.2(25 ℃) |
Subculture procedure | ① According to the culture conditions, the bacteria are first cultured into a bacterial suspension; ② Measure the absorbance of the bacterial suspension and calculate the initial concentration of the bacterial suspension according to the standard formula; The initial concentration multiplied by the survival rate is the actual concentration after freeze-drying; ④ According to the actual concentration after freeze-drying, the bacterial suspension was diluted to obtain the target concentration, and then subpacked and freeze-dried; |
Growth conditions | 30 ℃;24-48h; aerobic; |
Storage conditions | -80 ℃ |
Safety level | 1 |
morphology | The colony is 1-2mm in diameter, round, with neat edges, opaque, Milky White on the front, Milky White on the back, raised in the middle, smooth surface, moist texture, bright surface, easy to provoke, G + (blue-purple), cocci, purity: pure |
Sharing mode | Public welfare sharing |