quantitative strains of Staphylococcus epidermidis|BNCC353450 |BNCC

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Quantitative strains of staphylococcus epidermidis (Winslow and Winslow) Evans

Culture medium	Tryptone: 17.0, soy peptone: 3.0, sodium chloride: 5.0, dipotassium hydrogen phosphate: 2.5, glucose: 2.5g,pH:7.3±0.2(25 ℃)
Subculture procedure	① According to the culture conditions, the bacteria are first cultured into a bacterial suspension; ② Measure the absorbance of the bacterial suspension and calculate the initial concentration of the bacterial suspension according to the standard formula; The initial concentration multiplied by the survival rate is the actual concentration after freeze-drying; ④ According to the actual concentration after freeze-drying, the bacterial suspension was diluted to obtain the target concentration, and then subpacked and freeze-dried.
Growth conditions	37°C;18-24h; aerobic
Storage conditions	-80 ℃
Safety level	2
morphology	The colony is 0.5-1mm in diameter, round, neat edges, opaque, gray-white front, raised in the middle, bright surface, smooth surface, moist texture, G (blue-purple), cocci
Sharing mode	Public welfare sharing