Rabbit fibroid virus nucleic acid detection kit (fluorescent PCR method)|BNCC371949 |BNCC

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Rabbit fibroma virus nucleic acid detection kit (Fluorescence PCR method)

Culture medium	98%EMEM+2%FBS
Subculture procedure	1. Detection experiment: dissolve the components in the kit on ice, mix well, centrifuge for a short time and put them on ice for later use; 2. Reagent preparation: reaction system (25 μL), take 15 μL of reaction solution, add 2 μL of template, 8 μL of DEPC-treated water, mix well, and centrifuge briefly; 3. Reaction conditions: the first step is 95 ℃ for 1min, the second step is 95 ℃ for 10s, the third step is 58 ℃ for 30s, and the second to third steps are cycled 40 times. 4. Fluorescence channel: FAM;
Growth conditions	Culture temperature 37 ℃; Culture time 2-4 days; Gas environment 5% CO2 + 95% air;
Storage conditions	-20 ℃
Safety level	1
application	Rapid detection of rabbit fibroma virus
Sharing mode	Public welfare sharing