Lactobacillus rhamnosus nucleic acid detection kit (fluorescent PCR)|BNCC371945 |BNCC

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Lactobacillus rhamnosus nucleic acid detection kit (Fluorescence PCR method)

Culture medium	Peptone: 10.0, beef extract powder: 8.0, yeast powder: 4.0, glucose: 20.0, magnesium sulfate: 0.2, sodium acetate: 5.0, diammonium hydrogen citrate: 2.0, dipotassium hydrogen phosphate: 2.0, manganese sulfate: 0.04, Tween 80:1.0, agar: 14.0,pH:5.7±0.2(25 ℃)
Subculture procedure	1. Detection experiment: dissolve the components in the kit on ice, mix well, centrifuge for a short time and put them on ice for later use; 2. Reagent preparation: reaction system (25 μL), take 15 μL of reaction solution, add 2 μL of template, 8 μL of DEPC-treated water, mix well, and centrifuge briefly; 3. Reaction conditions: the first step is 95 ℃ for 1min, the second step is 95 ℃ for 10s, the third step is 58 ℃ for 30s, and the second to third steps are cycled 40 times. 4. Fluorescence channel: FAM;
Growth conditions	37 ℃,24-48h,5% CO2 + 95% air
Storage conditions	-20 ℃, dark and refrigerated
Safety level	2
application	Rapid Detection of Lactobacillus rhamnosus
Sharing mode	Public welfare sharing