BeNa Culture Collection
Subculture procedure | Sample treatment: sample treatment shall be carried out in accordance with the GB4789.44-2022 National Food Safety Standard Vibrio vulnificus Detection; Experimental preparation: dissolve the various components in the kit on ice before use, mix slightly and centrifuge for a short time, and place them on ice for later use; Reagent preparation: experimental reaction system (25 μL),qPCR mixed reaction solution 16 μL, template 1 μL, sterile ddH2O8 μL; Reaction conditions: 95 ℃ 1min 1cycle;95 ℃ 10s, 59 ℃ 30s 40cycle; Fluorescence channel: FAM; |
Safety level | 0 |
application | It is suitable for auxiliary diagnosis of Vibrio vulnificus infection in water samples, suspected contaminated water, food and other samples. |
Sharing mode | Public welfare sharing |