BeNa Culture Collection
Subculture procedure | Sample processing: sample processing shall be carried out according to the National Standard for GB4789.44-2022 Food Safety Vibrio vulnificus Detection; Experimental preparation: the various components in the kit are used to dissolve on ice, slightly mixed and briefly centrifuged, and then placed on ice for later use; Reagent preparation: experimental reaction system (25 μL),qPCR mixed reaction solution 16 μL, template 1 μL, sterile ddH2O8μL; Reaction conditions: 95 ℃, 1min, 1cycle;95 ℃, 10s, 59 ℃, 30s, 40cycle; Fluorescence channel: FAM; |
Safety level | 0 |
application | It is suitable for auxiliary diagnosis of Vibrio vulnificus infection in water samples, suspected contaminated water, food and other samples. |
Sharing mode | Public welfare sharing |
Vibrio vulnificus nucleic acid test kit certificate
1. Product information
Sample name: Vibrio vulnificus nucleic acid detection kit (fluorescent PCR method)
Sample number: 361446
Sample batch: 220505
2. Product features
Reagent composition | reaction solution 240μL/tube, sterile ddH2O 500μL/tube, positive quality control 20μL/tube, negative quality control 20μL/tube |
traceability | CICC21615 Vibrio vulnificus, ATCC BAA-2163 Vibrio cholerae |
save and transport | -20 ℃, valid for 1 year, dry ice transportation |
applicable fields | Suitable for auxiliary diagnosis of Shigella infection in water-like feces, suspected contaminated water, food and other samples |
*Note: If you have any questions about this kit, please contact our center (BNCC) for help before use |
3. Instructions
Sample processing: sample processing in accordance with "GB4789.44-2022 National Food Safety Standard - Vibrio vulnificus Detection";
Experiment preparation: Dissolve the various components in the kit on ice before use, mix gently and centrifuge briefly, then place on ice for later use;
Reagent preparation: experimental reaction system (25 μL), 16 μL qPCR mixed reaction solution, 1 μL template, 8 μL sterile ddH2O
Reaction conditions: 95℃ 1min 1cycle; 95℃ 10s, 59℃ 30s 40cycles;
Fluorescence channel: FAM;
4. Notes
Negative: The sample has no exponential growth curve, or has a lower amplification curve, and the Ct value is >38;
Positive: The sample has a typical exponential growth curve, and the Ct value is ≤38.
5. Preservation and transportation
1. Dissolve the various components in the kit on ice before use, mix thoroughly and centrifuge briefly and place on ice for later use;
2. In order to ensure the sensitivity of the detection, it is recommended that the qPCR mixed reaction solution be frozen and thawed no more than 3 times;
3. Sample processing, reagent preparation, and sample addition should be carried out in different areas to avoid cross-contamination;
Henan Engineering Research Center of Industrial Microbiology
Website: www.bncc .org .cn Tel: 400-6699-833