Enterohemorrhagic Escherichia coli (EHEC) nucleic acid detection kit (fluorescent PCR method)|BNCC381666 |BNCC

BeNa Culture Collection

  • Escherichia coli EHEC nucleic acid detection kit (QPCR)-BNCC

Escherichia coli EHEC nucleic acid detection kit (QPCR)

  • Price: Contact
  • number:BNCC381666
  • Packing:Frozen storage tube; 50T;4 tubes/box
Essential Information Related Products
Escherichia coli EHEC nucleic acid detection kit (QPCR)
Subculture procedure 1. Experimental preparation: dissolve the various components in the kit on ice before use, mix them slightly and centrifuge them briefly and place them on ice for later use; 2. Sample treatment: sample preparation shall be carried out in accordance with the "GB4789.6-2016 Diarrhea Escherichia coli Detection; 3. Reagent preparation: experimental reaction system (25 μL),qPCR mixed reaction solution 23 μL, template 2 μL; 4. Reaction conditions: 95 ℃ 3min 1cycle;94 ℃ 10s, 55 ℃ 30s 40cycle; 5. Fluorescence channel: VIC;
Storage conditions -20 ℃
Safety level 0
application It is suitable for the auxiliary diagnosis of enterohemorrhagic Escherichia coli (EHEC) infection in water samples, suspected contaminated water, food samples, etc.
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