Foot-and-mouth disease virus type O/A nucleic acid detection kit (duplex fluorescent PCR method)|BNCC373697 |BNCC

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Foot and Mouth Disease O/A nucleic acid detection kit (Fluorescent PCR method)

Culture medium	Special peptone: 23.0, soluble starch: 1.0, sodium chloride: 5.0, agar: 10.0, defibrated sheep blood: 5% (added at 50 ℃),pH:7.3±0.2(25 ℃)
Subculture procedure	Experimental preparation: dissolve the various components in the kit on ice before use, mix slightly and centrifuge for a short time, and place them on ice for later use; Experimental reaction system (25 μL):qPCR mixed reaction solution 15 μL, template 2-10 μL, and the rest supplemented with water; Experimental reaction conditions: the first step is 96 ℃ for 10min, the second step is 95 ℃ for 10s, the third step is 57 ℃ for 40s, and the second to third steps are cycled 40 times; Fluorescence Channel: FAM.
Growth conditions	culture temperature 37 ℃; culture time 72 hours; gas environment anaerobic;
Storage conditions	-20 ℃; protected from light
Safety level	2
Separation substrate	human feces
application	Suitable for foot-and-mouth disease virus type O/A nucleic acid detection
Sharing mode	Public welfare sharing