Bacillus licheniformis nucleic acid detection kit (fluorescent PCR method)|BNCC372367 |BNCC

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Bacillus licheniformis nucleic acid detection kit (Fluorescence PCR method)

Culture medium	Ammonium chloride: 1.0, potassium dihydrogen phosphate: 0.5, magnesium chloride: 0.5, calcium chloride: 0.1, sodium chloride: 1.0, sodium acetate: 2.0, sodium succinate: 1.0, yeast extract powder: 0.5, peptone: 0.5, ferrous chloride: 1.8mg, cobalt chloride: 0.25mg, nickel chloride: 0.01mg, copper chloride: 0.01mg, manganese chloride: 0.07mg, zinc chloride: 0.1mg, boric acid: 0.5mg, sodium selenite: 0.01mg, sodium molybdate: sodium molybdate: 0.01g, biotin: 0.01mg, nicotinic acid: 0.035mg, calcium pantothenate: 0.01mg, thiamine hydrochloride: 0.03mg,VB12:0.005mg, pyridoxamine hydrochloride: 0.01mg, p-aminobenzoic acid: 0.02mg, agar: 13.0,pH:6.8(25 ℃)
Subculture procedure	1. Experimental preparation: dissolve the components in the kit on ice, mix well, centrifuge for a short time and put them on ice for later use; 2. Sample processing: After the positive quality control is dissolved, it is then placed on ice for later use; 3. Reagent preparation: reaction system (25 μL), take 15 μL of reaction solution, add 2 μL of template and 8 μL of sterile ultrapure water, mix well and centrifuge for a short time; 4. Reaction conditions: the first step is 95 ℃ for 1min, the second step is 95 ℃ for 10s, the third step is 63 ℃ for 30s, and the second to third steps are cycled 40 times. 5. Fluorescence channel: FAM;
Growth conditions	culture temperature 30 ℃; culture time 18-24 hours;
Storage conditions	-20 ℃, dark and refrigerated
Safety level	2
Separation substrate	Human lesion
application	For rapid detection of Bacillus licheniformis
Sharing mode	Public welfare sharing