Vibrio parahaemolyticus (VP) nucleic acid detection kit (fluorescent PCR method)|BNCC361343 |BNCC

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Vibrio parahaemolyticus (VP) nucleic acid detection kit (Fluorescent PCR method)

Subculture procedure	1. Experimental preparation: dissolve the various components in the kit on ice before use, mix them slightly and centrifuge them briefly and place them on ice for later use; 2. Sample treatment: sample treatment shall be carried out in accordance with SN/T 5125-2019 Inspection of Vibrio parahaemolyticus; 3. Reagent preparation: experimental reaction system (25 μL),qPCR mixed reaction solution 21 μL, template 4 μL; 4. Reaction conditions: 95 ℃ 3min 1cycle;94 ℃ 10s, 52 ℃ 30s 40cycle; 5. Fluorescence channel: FAM;
Storage conditions	-20 ℃
Safety level	1
application	It is suitable for auxiliary diagnosis of Vibrio parahaemolyticus infection in water sample stool, suspected contaminated water, food and other samples
Sharing mode	Public welfare sharing