Bacillus cereus nucleic acid detection kit (fluorescence PCR method)|BNCC372298 |BNCC

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Bacillus cereus nucleic acid detection kit (Fluorescence PCR method)

Culture medium	Peptone: 10.0, beef powder: 3.0, sodium chloride: 5.0, agar: 15.0,pH: 7.3±0.1(25 ℃)
Subculture procedure	1. Experimental preparation: dissolve the components in the kit on ice, mix well, centrifuge for a short time and put them on ice for later use; 2. Sample processing: self-prepared or pre-extracted test sample template DNA; 3. Reagent preparation: reaction system (25 μL), take 15 μL of reaction solution, add 1 μL of template and 9 μL of DEPC water, mix well, and centrifuge briefly; 4. Reaction conditions: 95 ℃ 1min 1cycle;95 ℃ 30s, 52 ℃ 30s ,40cycle; 5. Fluorescence channel: FAM; Fluorescence Channel: FAM.
Growth conditions	culture temperature 37 ℃; culture time 20 hours; gas environment aerobic;
Storage conditions	-20 ℃, dark and refrigerated
Safety level	2
application	For rapid detection of Bacillus cereus
Sharing mode	Public welfare sharing