Murine pox virus nucleic acid detection kit (fluorescent PCR method)|BNCC363563 |BNCC

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Culture medium	98%EMEM+2%FBS
Subculture procedure	Experimental preparation: dissolve the various components in the kit on ice before use, mix slightly and centrifuge for a short time, and place them on ice for later use; Experimental reaction system (25 μL):qPCR mixed reaction solution 15 μL, template (1-10)μL, sterile ddH2O to 25 μL; Experimental reaction conditions: the first step is 95 ℃ for 1min, the second step is 95 ℃ for 10s, the third step is 54 ℃ for 30s, and the second to third steps are cycled 40 times; Fluorescence Channel: FAM.
Growth conditions	Culture temperature 37 ℃; Culture time 5 days; Gas environment 5% CO2 + 95% air;
Storage conditions	-20 ℃, protected from light
Safety level	1
application	Suitable for in vitro amplification of mouse pox virus detection, can not be used for clinical diagnosis of disease
Sharing mode	Public welfare sharing